ANTIBACTERIAL ACTIVITY OF ADHATODA VASICA PDF

Adhatoda vasica belonging to Acanthaceae family, commonly known as Adosa, Adhatoda vasica Flowers, Antibacterial activity, Antifungal activity, Diffusion. Abstract. The present study aimed to evaluate the in vitro antimicrobial activity of pharmacologically important Adhatoda vasica plant extracts. Antibacterial activity of leaf extract of Adhatoda vasica was studied against Bacillus subtilis, Antibacterial activity was determined by disc diffusion method.

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Vasicine acetate exhibited qctivity zone of inhibition against bacteria: Vasicine acetate could be probed further in drug discovery programme.

The radical scavenging activity of Vasicine acetate at different concentrations is shown in Table 3.

UV-Visible spectra were taken in methanol on Thermo Fisher instrument. Introduction Natural products still remain the most important source for discovery of new and potential drug molecules. Vasicine is reported to have bronchodilatory, respiratory od, and uterine stimulant effects [ 14 ].

A human adenocarcinoma cancer cell line was obtained from National Institute of Cell Sciences, Pune. The spot turned orange red on spraying with Dragendorff’s reagent.

Antibacterial activity of adhatoda vasica leaf extract

The structure was elucidated using spectroscopic methods: Journal of Natural Products. DPPH 2,2-diphenylpicrylhydrazyl radical scavenging activity of Vasicine acetate was determined based on the method of Wang et al. The physical and spectroscopic data were compared with those reported in the literature [ 2122 ].

The extract was filtered through Whatman No. The plant contains alkaloids such as Vasicine, vasicinone, antibacteerial, vasicol, activitt, and vasicinol [ 12 ]. Vasicine acetate showed minimum inhibitory concentration values against bacteria; they were M.

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Minimum inhibitory concentration studies of the compounds were performed according to the standard reference methods for bacteria [ 17 ] and filamentous fungi [ 18 ].

Streptomycin was used as positive control for bacteria and Ketoconazole as positive control for fungi. Support Center Support Center.

Ignacimuthu123 C. Vasicine acetate was obtained by acetylation of Vasicine recovered from A. Conflict of Interests The authors declare that there is no conflict of interests regarding the publication of this paper. Negative control was prepared using respective solvents. BHT is used as standard of antioxidant. Then the residue was crystallised from chloroform to get Vasicine acetate as colorless crystals m. National Center for Biotechnology InformationU.

Cytotoxic A and antimicrobial effects of Methylobacterium sp. Published online Jan Separation was carried out adharoda room temperature. It was then distilled on a water bath and the residue was washed with little n-hexane.

In vitro antibacterial activity of selected medicinal plants from lower Himalayas.

Journal List Biomed Res Int v. Sample ethanol extract Medicinal plants are important sources of practical drugs for people throughout the year.

The free radical scavenging activity was calculated as follows: Microbial Organisms The following Gram negative, Gram positive bacteria, clinical isolates, and fungi were used for the experiment: The physical and spectroscopic data were compared with those reported in the literature [ 15 ]. Vasicine hydrochloride, a new drug for interruption of pregnancy. Escherichia coli was highly resistant to all the plant extracts at all concentrations.

Materials and Methods 2.

Antibacterial Activity of Leaf Extracts of Adhatoda vasica – MAHE Digital Repository

Adhatoda vasica, Bauhenia variegate, Bombax ceiba, Carrisa opaca, Caryopteris grata, Debregeasia salicifolia, Lantana camara, Melia azedarach, Phyllanthus emblica, Pinus roxburghii and Olea ferruginea collected from lower Himalayas against two Gram positive Staphylococcus aureus, Micrococcus luteus and two Gram negative Escherichia coli, Pseudomonas aureginosa bacterial strains.

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Effect of Adhatoda vasica massage in pyorrhoea. The cytotoxicity was determined according to the method of Balachandran et al. A voucher specimen No. Nature acts as a prominent reservoir for new and novel therapeutics. The required concentrations, fo, HPLC analysis was carried out on a Waters Alliance separations module with photodiode array detector Waters, Clinical and Laboratory Standards Institute; Antifeedant and toxic activity of Adhatoda vasica leaf extract against Spodoptera littoralis Lep.

The present studies cover antibacterial activity of the crude methanolic extracts of 11 medicinal plants viz. Indian Journal of Medical Research.

Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

The MIC for bacteria was determined as the lowest concentration of the compound inhibiting the visual growth of the test cultures on the agar plate. The alkaloid Vasicine was isolated from ethanolic extract of the leaves of A.

Classical Uses of Medicinal Plants. Antitussive effect of Adhatoda vasica extract on mechanical or chemical stimulation-induced coughing in animals. Cytotoxic studies against A lung adenocarcinoma cancer cell line showed Vasicine acetate to be the most potent compound Table 4.

Essential oils of the leaves of A.